How To Making Spore Syringe Without Pressure Cooker. You will also need sterilized water, a gas burner, a pressure cooker and other materials. Sterilize the flask or glass bottle use a flask or a glass bottle with a narrow opening to reduce the chances of contamination or microbes getting inside it.
I'd do a little trial with the water amount. Remove the syringe and flip down the micropore tape. With the needle still hot inject 1 cc into each jar, splitting it between two of the holes.
Shake needle to spread out current mycelium.
Drop a single drop of spore solution onto the agar. Thoroughly wipe down the with alcohol, then spray the entire area liberally. Shake the syringe well, then insert the needle all the way into one of the holes. Dip the tip of the syringe without the needle and pull the plunger back to suck in the water along with the spore suspensions.